Beneficial Therapeutic Impact of Berberine and Donepezil on Neurotoxicity in Rats

Document Type : Research and Reference

Authors

1 Department of Biochemistry ,Faculty of Science, Damanhour University, Egypt

2 Professor of Biochemistry , Chemistry Department , Tanta university , Tanta

Abstract

Alzheimer's disease (AD) is a neurological ailment is marked by cognitive impairment, oxidative stress, and damage to neurons.  Berberine chloride (BBR) is an antioxidant supplement that can help with oxidative stress that comes from brain injury.  This study sought to investigate the therapeutic potential of BBR.  It also aimed to identify any synergistic effects when paired with donepezil treatment on cerebral injury in rats.  We set up seven experimental groups: control group that got saline (GI), control group that got berberine (GII), control group that got donepezil (GIII), AlCl₃/D-galactose-induced AD group (GIV), AD group that got berberine (GV), AD group that got donepezil (GVI), and AD group that got both berberine and donepezil (GVII).  We looked at biochemical markers such catalase activity, levels of noradrenalin, liver and kidney function, lipid profile, and calcium levels. A histopathological study was conducted to assess neuronal integrity.  The AD model group showed significant decrease in catalase, noradrenalin, albumin and HDL as well as significant increase in liver enzymes, urea, creatinine and calcium concentration with serious damage to neurons in the hippocampus.  Berberine or donepezil alone partially corrected these changes, making the antioxidant defense, neurotransmitter balance, and tissue morphology better.  The combination therapy notably yielded the most significant reinstating biochemical and morphological change in hippocampus beyond the effects of either treatment alone. In conclusion, BBR exhibited significant neuroprotective and its conjunction with donepezil had synergistic effects against Alzheimer's disease-like pathology. These findings indicate that berberine presenting a viable approach for the management of AD. 

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