The possible ameliorative role of Phoenix dactylifera seed extracts against hepatotoxicity induced by Acetaminophen in rats

Document Type : Research and Reference

Authors

Histoloy, Zoology department, Faculty of science, Tanta university, Tanta, Egypt

Abstract

Acetaminophen, known commercially as paracetamol, is used as a pain reliever and antipyretic drug. It is very safe if taken in appropriate doses (4 grams/ day), but when taken in excessive dosages, it may lead to some health problems. This work aims to establish the possible protective role of Phoenix dactylifera seed extracts against hepatotoxicity that may be caused by acetaminophen in rats. The bioactive compounds of P. dactylifera seeds have been identified using gas chromatography-mass spectrometry (GC/MS). Rats were divided into three groups of ten rats each: Gp1 (the -ve control group); Gp2 (+ve control group), which received acetaminophen orally (750 mg/kg daily); and Gp3 was injected with P. dactylifera seed extracts (200 mg/kg) after acetaminophen ingestion for a month (750 mg/kg daily). During this work, the initial and final rat body weights, hepatic function tests, and the antioxidant enzyme activities were measured; also, histological and immunohistochemical studies were examined. GC-MS analysis results showed the presence of various phytochemical components with potent antioxidants and anti-inflammatory activities. Administration of acetaminophen resulted in a significant decrease in the rats' final body weight. In contrast, treatment of rats by P. dactylifera extracts displayed a small percentage increase in their body weight. For biochemical tests, acetaminophen administration caused significant alterations in hepatic functions and antioxidant enzymes, while the group treated with P. dactylifera improved both. Histological and immunohistochemical staining supported these results. In conclusion, treatment with p. dactylifera seed extracts showed a potent therapeutic and protective effect against acetaminophen hepatotoxicity in rats.

Keywords

Main Subjects

Files

Share

How to cite

Statistics