Chemical characterization, fumigant toxicity and antifeedant activity of essential oils of four indigenous plants against Rhyzopertha dominica (Coleoptera: Bostrychidae)

Seada, Mervat A.; Hamza, Amal Mustafa; Abouelatta, Ahmed Mohamed

doi:10.21608/djs.2024.252136.1141

Chemical characterization, fumigant toxicity and antifeedant activity of essential oils of four indigenous plants against Rhyzopertha dominica (Coleoptera: Bostrychidae)

Document Type : Research and Reference

Authors

¹ Entomology Division, Zoology Department, Faculty of Science, Tanta University, Egypt

² Stored Product Pests Department, Plant Protection Research Institute, Agriculture Research Center, Giza 12611, Egypt

³ Stored product pests, Plant protection institute, Agriculture research center, Egypt

10.21608/djs.2024.252136.1141

Abstract

Plant-based essential oils (EOs) have been widely explored as biocontrol agents against stored product insects due to their complex mixtures of volatile compounds that often possess insecticidal, or antifeedant activities. Thus, this study aimed to investigate the fumigant, and antifeedant activity of four EOs extracted from violet (Viola odorata), neroli (Citrus aurantium), parsley (Petroselium crispum), and marjoram (Origanum majorana) against the lesser grain borer, Rhyzopertha dominica. Based on gas chromatography/mass spectrometry (GC-MS) analysis, the principal compounds detected were linolenic acid (60.014%), α-Linolenate (16.01%), and palmitic acid (15.58%) in the EO of V. odorata. Linalyl acetate (46.09%), 1,6-octadiene, and β-linalool (26.94%) in EO of C. aurantium, and 1,3,8-p-menthatriene (23.3%), α-pinene (13.0%), apiol (12.71%), and 1,3-benzodioxole (11.67%) in EO of P. crispum. 3-cyclohexene (26.12%), γ-terpinene (12.96%), α-terpinene (8.74%), and linalool (8%) in the EO of O. majorana. Essential oil of O. majorana was the premier fumigant based on the LC50 values (643 and 412.9 mg/L) and toxicity index (100%), while V. odorata oil showed the lowest effect as it achieved LC₅₀ values of (2384.4 and 2189.2 mg/L) and toxicity indices of (26.96% and 18.86%) after 3 and 6 h postexposure, respectively. Furthermore, the tested EOs displayed feeding deterrent indices, 46.67%, 78.67%, 54.66%, and 88% with the higher concentrations of V. odorata, C. aurantium, P. crispum, and O. majorana, respectively. Results indicated that the tested EOs achieved satisfactory biological activities, enabling them to be used as perfect natural ingredients in toxicity and deterring feeding of R. dominica.

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