Document Type : Research and Reference
Authors
1
1Biochemistry division, Chemistry department, Faculty of Science, Tanta University, Tanta, Egypt.
2
Biochemistry division, Chemistry department, Faculty of Science, Tanta University, Tanta, Egypt.
3
Biochemistry Division, Chemistry department, Faculty of Science, Tanta University, Tanta, Egypt.
Abstract
Cyclooxygenases (EC 1.14.99.1) is enzyme family that produces inflammatory prostaglandins, also known as
prostaglandin H synthase (PGHS) or prostaglandin-endoperoxide synthase, is expressed in several tissues. COX activity has been
detected in both brain and kidney of mice and bovine kidney (that showed the highest activity). Optimum biochemical properties
of enzyme activity including incubation time, enzyme and substrate concentrations and pH were determined. Kinetic parameters
of COX activity were indicated that the Vmax and Km values were of 66.66 μmole/min and 125.73 μM-1, respectively. COX has
been purified from bovine kidney by detergent solubilization, (NH4)2SO4 precipitation, dialysis and ion exchange chromatography
on DEAE-sepharose. Three isoforms were obtained (coxI, coxII and coxIII) that showed specific activities of 592.27, 1323.3 and
1825 U/mg protein and 0.72, 1.6 and 2.2 folds purification over the crude homogenate, respectively. The purified enzyme
exhibited a single protein band on Coomassie Brilliant Blue stained SDS-PAGE gel corresponding a molecular weight 70 KDa.
Keywords
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