Cytogenetic studies on five Red Sea reef fish species Larabicus quadrilineatus, Hemigymnus melapterus, Thalassoma rueppellii, Thlassoma lunare and Gomphosus caeruleus of the family Labridae (Perciformes) were carried out; all samples were collected from Hurghada, on the Egyptian Red Sea coast. All the specimens showed a diploid chromosome number of 2n=48, they were also identical in the karyotype (all acrocentric), and in the Fundamental number FN=48. The RAPD-PCR analysis were carried out by using eight primers OPA12, 5`-TCGGCGATAG-3, OPA14,5`-TCTGTGCTGG-3`, OPA15, 5`-TTCCGAACCC- 3`, OPA17,5`- GACCGCTTGT – 3`, OPA18 5`-AGGTGACCGT-3`, OPA19 5`-CAAACGTCGG- 3`,OPA20,5`-GTTGCGATCC-3` and OPO11,5`- GACAGGAGGT- 3`. The results were extremely useful to improve the importance of using RAPD-PCR in genetic analysis among the species which are identical in chromosome number and karyotype.
M. Ebied, A., Abu-El-Regal, M. A., Abu-Almaaty, A. H., & Mar`ie, Z. A. (2013). Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef. Delta Journal of Science, 36(2), 68-84. doi: 10.21608/djs.2013.139656
MLA
Abdel-Basset M. Ebied; M. A Abu-El-Regal; A. H Abu-Almaaty; Zeinab A Mar`ie. "Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef", Delta Journal of Science, 36, 2, 2013, 68-84. doi: 10.21608/djs.2013.139656
HARVARD
M. Ebied, A., Abu-El-Regal, M. A., Abu-Almaaty, A. H., Mar`ie, Z. A. (2013). 'Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef', Delta Journal of Science, 36(2), pp. 68-84. doi: 10.21608/djs.2013.139656
VANCOUVER
M. Ebied, A., Abu-El-Regal, M. A., Abu-Almaaty, A. H., Mar`ie, Z. A. Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef. Delta Journal of Science, 2013; 36(2): 68-84. doi: 10.21608/djs.2013.139656
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