Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients

Document Type : Research and Reference

Authors

1 Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq.

2 Baghdad University, Science college, Biotechnology

3 engineering and biotechnology institute, Baghdad University

4 University Kebangsaan Malaysia, Faculty of Science and Technology, School of Bioscience

Abstract

This work aimed to study the possible mutations in Perforin gene (PRF1) exon 2-3 and Fas
gene exon 4 in Acute Lymphocytic Leukemia (ALL) and Chronic Lymphocytic Leukemia
(CLL) patients from Iraq. In an attempt to detect any mutation within PRF1 gene and Fas genes,
a sequencing analysis for these genes were made. The results were alignment with sequences
present in the Gene Bank seeking for homology and differences. A DNA sequence for Homo
sapiens PRF1 gene was found compatible with genes of ALL, CLL patients and healthy
controls, 100% compatibility was found in the flank DNA sense and antisense sequences from
healthy. However, 99% compatibility was detected for the genes isolated from ALL patients
with an insertion of C697 and A698 G in the flank DNA sense strand and insertion of G697 and
T698C in flank DNA antisense strand of the gene. Morever, 99% compatibility was detected for
the genes isolated from CLL patients with two transition mutations in the flank DNA sense
strand of C957T and C1035T and one transition mutation in the flank DNA antisense strand of
G957A. However, no mutations were detected in Fas gene isolated from ALL, CLL, and healthy
controls.

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