Investigation in the roots compositions of the Pimpinella boissieri M. Heroe grown wild in Kurdistan – Iraq using GC and LC-MS

Document Type : Research and Reference

Authors

1 Division of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.

2 Division of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK

3 Food Science Department, Faculty of Agricultural Sciences, University of Sulaimani, KRG, Iraq

Abstract

Many plant root extracts are known for their potential medicinal uses on living systems due to
one or more of their active compounds. In this study, the root’s composition of a rare species of
pimpinella “Pimpinella boisseiri” grown in wild in Kurdistan – Iraq was investigated after being
freeze dried, ground, extracted and purified. The purified ethanolic extract was analysed by LCMS
and two chlorogenic acids were identified including 3, 4, 5, - Caffeoylquinic acid, 3,4
DicaffeolyQuinic Acid and 3, 5-Feruloyl Quinic Acid. Hydro-distillation extract of the ground
roots resulted in obtaining azulene (0.5 %). This characterization of pimpinella root extracts
revealed the presence of the chlorogenic acids and Azulene. On SEM analysis with built-in XRay
analysis for the endosperm and the outer coat of the roots, the former showed to contain
Mg, P, S, and K, whereas the outer coat found to contain calcium and to a lesser extend iron,
beside the elements found in the endosperm.

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